A Calibration is one of the key processes to design and implement of Bioelectrical Impedance Spectroscopy. Through the simulation, the specification of hardware can be determined and examined in the ideal condition. After the implementation of the device, the theoretical and measured values are different due to the uncertainties and noises from the environment. In this paper, the calibration procedure will be introduced and discussed for the different sets of electrodes. By adjusting the different values of the active electrodes and applying for the active shields, the measured value will be compared and discussed. Active electrodes and active shields will be a very effective way to isolate and minimize the noises and avoid unwanted stray capacitance values. Further investigations need to be followed to ensure the proposed way to reduce the noises and stray capacitances.