Microvascular injury is recognized as a major tissue damage mechanism of ablative cryosurgery. Details of this injury mechanism are not completely understood. But it is known that extracellular ice propagating through the vascular region leads to endothelial cell dehydration, which may cause their detachment from each other and eventually from the vessel wall. Soon after post-thaw reperfusion vessel leakage is evident and thrombi form in the vessels leading to vascular stasis and consequently tissue ischemia. To better understand the mechanical principles underlying this tissue injury mechanism, we have modeled water transport phenomena that arise during the freezing of the vasculature. Endothelial cells were modeled as an independent but connected array of ellipsoidal balloons whose size varies according to the osmotic pressure experienced during the freezing process. An assumption of minimum surface area permeable to water was made as the mechanism governing the cell shape change. Variations in intercellular gap dimensions under different freezing protocols were obtained numerically. The results were then compared to the observed behavior of cultured endothelial cells undergoing the same freezing protocol on a cryostage. The simulation and experimental results are in good agreement.
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ASME 2003 International Mechanical Engineering Congress and Exposition
November 15–21, 2003
Washington, DC, USA
Conference Sponsors:
- Heat Transfer Division
ISBN:
0-7918-3718-1
PROCEEDINGS PAPER
Study of Vascular Endothelium Damage Due to Freezing
Aili Zhang,
Aili Zhang
Purdue University, West Lafayette, IN
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Lisa X. Xu,
Lisa X. Xu
Purdue University, West Lafayette, IN
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George A. Sandison
George A. Sandison
Purdue University, West Lafayette, IN
Search for other works by this author on:
Aili Zhang
Purdue University, West Lafayette, IN
Lisa X. Xu
Purdue University, West Lafayette, IN
George A. Sandison
Purdue University, West Lafayette, IN
Paper No:
IMECE2003-41967, pp. 417-420; 4 pages
Published Online:
May 12, 2008
Citation
Zhang, A, Xu, LX, & Sandison, GA. "Study of Vascular Endothelium Damage Due to Freezing." Proceedings of the ASME 2003 International Mechanical Engineering Congress and Exposition. Heat Transfer, Volume 4. Washington, DC, USA. November 15–21, 2003. pp. 417-420. ASME. https://doi.org/10.1115/IMECE2003-41967
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