Recent research suggests that estradiol affects the material properties of the Anterior Cruciate Ligament (ACL). Here we experimentally determine the cross-sectional area and tensile properties of ACLs from the groups of prepubertal rabbits: untreated control (C), estrogen treated (E) and estrogen and epidermal growth factor treated (E+EGF). Estradiol decreased the ultimate tensile strength and toughness of the ACL. Addition of EGF decreased the modulus of elasticity.

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