Dielectrophoresis (DEP) has been demonstrated as an effective mechanism for cell sorting in microfluidic settings. Many existing methods utilize sophisticated microfluidic designs that require complicated fabrication process and operations. In this paper, we present a microfluidics-based cell sorter that is capable of sorting microparticles continuously in a simple straight channel, thus facilitating easier fabrication and operation. An array of indium-tin oxide (ITO) electrodes are embedded on the bottom surface of the straight channel to generate a DEP force field. This force results in deviation of the particles with different dielectric properties from their paths that are hydrodynamically focused in the channel. Particle trajectories are predicted by numerical simulation at different flow rates and field strengths using COMSOL. Separation of red blood cells from polystyrene beads is demonstrated and numerical prediction is validated experimentally. High separation efficiency for the two particle types is confirmed by counting the concentrations of particles collected at the respective collection outlet.

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