Endothelial cells line on the inner surface of the vascular is continuously exposed to shear stress by a blood flow in vivo. It is known that shear stress influences energy-dependent physical (Dewey et al., 1981, Levesque et al., 1985) and biochemical characteristics of endothelial cells (Arisaka et al., 1995). We, therefore, evaluated mitochondrial ATP synthesis activity by a confocal laser scanning microscope (CLSM) and a mitochondrial membrane potential probe, 5.5’, 6.6’-tetrachloro-1.1’,3,3’-tetrathylbenzimidazolocarbocyananine iodide (JC-1), when shear stress was loaded over cultured endothelial cells.

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