Back pain is a significant clinical concern often attributed to degeneration of the intervertebral disc (IVD) and the associated dehydration of the nucleus pulposus (NP) . The NP is a gel-like tissue at the center of the disc, rich in proteoglycans and type II collagen that functions to resist compressive forces through the generation of a hydrostatic swelling pressure . Tissue engineering strategies may provide a viable NP replacement therapy as an alternative to current surgical procedures. However, several factors including medium formulation and scaffold selection can affect construct maturation . For example, transforming growth factor-beta 3 (TGF-β3) has been shown to enhance the functional properties of tissue engineered cartilage constructs, with more pronounced results observed in serum-free conditions . NP cells are commonly cultured in ionically crosslinked alginate hydrogels to maintain their phenotypic properties; however, these scaffolds have been shown to lose structural integrity over time, creating a need for an alternative biomaterial . Therefore, the objective of this study was to examine the effects of medium formulation on NP cells encapsulated in novel photocrosslinked carboxymethylcellulose (CMC) hydrogels.
- Bioengineering Division
Chemically Defined Medium With TGF-β3 Enhances Matrix Elaboration by Nucleus Pulposus Cells Encapsulated in Novel Photocrosslinked Carboxymethylcellulose Hydrogels
- Views Icon Views
- Share Icon Share
- Search Site
Reza, AT, & Nicoll, SB. "Chemically Defined Medium With TGF-β3 Enhances Matrix Elaboration by Nucleus Pulposus Cells Encapsulated in Novel Photocrosslinked Carboxymethylcellulose Hydrogels." Proceedings of the ASME 2009 Summer Bioengineering Conference. ASME 2009 Summer Bioengineering Conference, Parts A and B. Lake Tahoe, California, USA. June 17–21, 2009. pp. 1025-1026. ASME. https://doi.org/10.1115/SBC2009-206199
Download citation file: