Background: During conventional cryopreservation, cells are often damaged by ice formation. An alternative approach is to use ultra-rapid cooling rates to achieve vitrification of cell suspension. A variety of systems and methods have been developed, e.g. open pulled straws (OPS), closed pulled straws (CPS), cryo-loop and micro-droplets, by which cooling/warming rates up to 104∼105 °C/min can be achieved. Although these methods have been well used in some areas, they are still far from perfect because (1) the cooling/warming rates are still not fast enough and thus high-concentration cryoprotectants should be used; (2) the sample size is often seriously limited.
- Bioengineering Division
Numerical Investigation of a Novel Microfluidic System and its Application in Achieving Ultra-Fast Cooling/Warming Rates for Cell Vitrification Cryopreservation
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Zhou, X, Liang, X, Shu, Z, Du, P, & Gao, D. "Numerical Investigation of a Novel Microfluidic System and its Application in Achieving Ultra-Fast Cooling/Warming Rates for Cell Vitrification Cryopreservation." Proceedings of the ASME 2013 Summer Bioengineering Conference. Volume 1A: Abdominal Aortic Aneurysms; Active and Reactive Soft Matter; Atherosclerosis; BioFluid Mechanics; Education; Biotransport Phenomena; Bone, Joint and Spine Mechanics; Brain Injury; Cardiac Mechanics; Cardiovascular Devices, Fluids and Imaging; Cartilage and Disc Mechanics; Cell and Tissue Engineering; Cerebral Aneurysms; Computational Biofluid Dynamics; Device Design, Human Dynamics, and Rehabilitation; Drug Delivery and Disease Treatment; Engineered Cellular Environments. Sunriver, Oregon, USA. June 26–29, 2013. V01AT20A005. ASME. https://doi.org/10.1115/SBC2013-14077
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