Mechanical deformation of cell-seeded electrospun matrices plays an important role in cell signaling. However, electrospun biomaterials have inherently complex geometries due to the random deposition of fibers during the electrospinning process. This confounds attempts at quantifying strains exerted on adherent cells during electrospun matrix deformation. We have developed a novel mechanical test platform that allows deposition and tensile testing of electrospun fibers in a highly parallel arrangement to simplify mechanical analysis of the fibers alone and with adherent cells. The device is capable of optically recording fiber strain in a cell culture environment. Here we report on the mechanical and viscoelastic properties of highly parallel electrospun poly(ε-caprolactone) fibers. Force-strain data derived from this device will drive the development of cellular mechanotransduction studies as well as the customization of electrospun matrices for specific engineered tissue applications.
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Technical Briefs
References
1.
Baker
, B. M.
, Nerurkar
, N. L.
, Burdick
, J. A.
, Elliott
, D. M.
, and Mauck
, R. L.
, 2009, “Fabrication and Modeling of Dynamic Multipolymer Nanofibrous Scaffolds
,” J. Biomech. Eng.
, 131
(10
), p. 101012
.2.
Phipps
, M. C.
, Clem
, W. C.
, Catledge
, S. A.
, Xu
, Y.
, Hennessy
, K. M.
, Thomas
, V.
, Jablonsky
, M. J.
, Chowdhury
, S.
, Stanishevsky
, A. V.
, Vohra
, Y. K.
, and Bellis
, S. L.
, 2011, “Mesenchymal Stem Cell Responses to Bone-Mimetic Electrospun Matrices Composed of Polycaprolactone, Collagen I and Nanoparticulate Hydroxyapatite
,” PLoS ONE
, 6
(2
), p. e16813
.3.
Amoroso
, N. J.
, D’Amore
, A.
, Hong
, Y.
, Wagner
, W. R.
, and Sacks
, M. S.
, 2011, “Elastomeric Electrospun Polyurethane Scaffolds: The Interrelationship Between Fabrication Conditions, Fiber Topology, and Mechanical Properties
,” Adv. Mater.
, 23
(1
), pp. 106
–111
.4.
Courtney
, T.
, Sacks
, M. S.
, Stankus
, J.
, Guan
, J.
, and Wagner
, W. R.
, 2006, “Design and Analysis of Tissue Engineering Scaffolds That Mimic Soft Tissue Mechanical Anisotropy
,” Biomaterials
, 27
(19
), pp. 3631
–3638
.5.
D’Amore
, A.
, Stella
, J. A.
, Wagner
, W. R.
, and Sacks
, M. S.
, 2010, “Characterization of the Complete Fiber Network Topology of Planar Fibrous Tissues and Scaffolds
,” Biomaterials
, 31
(20
), pp. 5345
–5354
.6.
Stella
, J. A.
, D’Amore
, A.
, Wagner
, W. R.
, and Sacks
, M. S.
, 2010, “On the Biomechanical Function of Scaffolds for Engineering Load-Bearing Soft Tissues
,” Acta Biomater.
6
(7
), pp. 2365
–2381
.7.
Driscoll
, T. P.
, Nerurkar
, N. L.
, Jacobs
, N. T.
, Elliott
, D. M.
, and Mauck
, R. L.
, 2011, “Fiber Angle and Aspect Ratio Influence the Shear Mechanics of Oriented Electrospun Nanofibrous Scaffolds
,” J. Mech. Behav. Biomed. Mater.
, 4
(8
), pp. 1627
–1636
.8.
Lake
, S. P.
, and Barocas
, V. H.
, 2011, “Mechanical and Structural Contribution of Non-Fibrillar Matrix in Uniaxial Tension: A Collagen-Agarose Co-Gel Model
,” Ann. Biomed. Eng.
, 39
(7
), pp. 1891
–1903
.9.
Mauck
, R. L.
, Baker
, B. M.
, Nerurkar
, N. L.
, Burdick
, J. A.
, Li
, W. J.
, Tuan
, R. S.
, and Elliot
, D. M.
, 2009, “Engineering on the Straight and Narrow: The Mechanics of Nanofibrous Assemblies for Fiber-Reinforced Tissue Regeneration
,” Tissue Eng. B Rev.
, 15
(2
), pp. 171
–193
.10.
Nerurkar
, N. L.
, Elliott
, D. M.
, and Mauck
, R. L.
, 2007, “Mechanics of Oriented Electrospun Nanofibrous Scaffolds for Annulus Fibrous Tissue Engineering
,” J. Orthop. Res.
, 25
(8
), pp. 1018
–1028
.11.
Raghupathy
, R.
, and Barocas
, V. H.
, 2010, “Generalized Anisotropic Inverse Mechanics for Soft Tissues
,” J. Biomech. Eng.
, 132
(8
), p. 081006
.12.
Raghupathy
, R.
, Witzenburg
, C.
, Lake
, S. P.
, Sander
, E. A.
, and Barocas
, V. H.
, 2011, “Identification of Regional Mechanical Anisotropy in Soft Tissue Analogs
,” J. Biomech. Eng.
, 133
(9
), p. 091011
.13.
Stella
, J. A.
, Liao
, J.
, Hong
, Y.
, David Merryman
, W.
, Wagner
, W. R.
, and Sacks
, M. S.
, 2008, “Tissue-to-Cellular Level Deformation Coupling in Cell Micro-Integrated Elastomeric Scaffolds
,” Biomaterials
, 29
(22
), pp. 3228
–3236
.14.
Stella
, J. A.
, Wagner
, W. R.
, and Sacks
, M. S.
, 2010, “Scale-Dependent Fiber Kinematics of Elastomeric Electrospun Scaffolds for Soft Tissue Engineering
,” J. Biomed. Mater. Res.
, 93
(3
), pp. 1032
–1042
.15.
Tan
, E. P. S.
, and Lim
, C. T.
, 2004, “Novel Approach to Tensile Testing of Micro- and Nanoscale Fibers
,” Rev. Sci. Instrum.
, 75
(8
), pp. 2581
–2585
.16.
Tan
, E. P. S.
, and Lim
, C. T.
, 2004, “Physical Properties of a Single Polymeric Nanofiber
,” Appl. Phys. Lett.
, 84
(9
), pp. 1603
–1605
.17.
Tan
, E. P. S.
, Goh
, C. N.
, Sow
, C. H.
, Lim
, C. T.
, 2005, “Tensile Test of a Single Nanofiber Using an Atomic Force Microscope Tip
,” Appl. Phys. Lett.
, 86
(7
), p. 073115
.18.
Tan
, E. P.
, Ng
, S. Y.
, and Lim
, C. T.
, 2005, “Tensile Testing of a Single Ultrafine Polymeric Fiber
,” Biomaterials
, 26
(13
), pp. 1453
–1456
.19.
Baker
, S.
, Sigley
, J.
, Helms
, C. C.
, Stitzel
, J.
, Berry
, J.
, Bonin
, K.
, and Guthold
, M.
, 2012, “The Mechanical Properties of Dry, Electrospun Fibrinogen Fibers
,” Mater. Sci. Eng.
, C, 32
, pp. 215
–221
.20.
Carlisle
, C. R.
, Coulais
, C.
, Namboothiry
, M.
, Carroll
, D. L.
, Hantgan
, R. R.
, and Guthold
, M.
, 2009, “The Mechanical Properties of Individual, Electrospun Fibrinogen Fibers
,” Biomaterials
, 30
(6
), pp. 1205
–1213
.21.
Croisier
, F.
, Duwez
, A. S.
, Jérôme
, C.
, Léonard
, A. F.
, van der Werf
, K. O.
, Dijkstra
, P. J.
, and Bennink
, M. L.
, 2012, “Mechanical Testing of Electrospun PCL Fibers
,” Acta Biomater
., 8
(1
), pp. 218
–224
.22.
Stylianopoulos
, T.
, Bashur
, C. A.
, Goldstein
, A. S.
, Guelcher
, S. A.
, Barocas
, V. H.
, 2008, “Computational Predictions of the Tensile Properties of Electrospun Fibre Meshes: Effect of Fibre Diameter and Fibre Orientation
,” J. Mech. Behav. Biomed. Mater.
, 1
(4
), pp. 326
–335
.23.
Balestrini
, J. L.
, Chaudhry
, S.
, Sarrazy
, V.
, Koehler
, A.
, and Hinz
, B.
, 2012, “The Mechanical Memory of Lung Myofibroblasts
,” Integr. Comp. Biol.
, 4
(4
), pp. 410
–421
.24.
Heo
, S. J.
, Nerurkar
, N. L.
, Baker
, B. M.
, Shin
, J. W.
, Elliott
, D. M.
, and Mauck
, R. L.
, 2011, “Fiber Stretch and Reorientation Modulates Mesenchymal Stem Cell Morphology and Fibrous Gene Expression on Oriented Nanofibrous Microenvironments
,” Ann. Biomed. Eng.
, 39
(11
), pp. 2780
–2790
.25.
Huang
, X.
, Yang
, N.
, Fiore
, V. F.
, Barker
, T. H.
, Sun
, Y.
, Morris
, S. W.
, Ding
, Q.
, Thannickal
, V. J.
, and Zhou
, Y.
, 2012, “Matrix Stiffness-Induced Myofibroblast Differentiation is Mediated by Intrinsic Mechanotransduction
,” Am. J. Respir. Cell Mol. Biol.
, 1
, pp. 1
–41
.26.
Kurpinski
, K.
, Chu
, J.
, Hashi
, C.
, and Li
, S.
, 2006, “Anisotropic Mechanosensing by Mesenchymal Stem Cells
,” Proc. Natl. Acad. Sci.
, 103
(44
), pp. 16095
–16100
.27.
Mann
, J. M.
, Lam
, R. H.
, Weng
, S.
, Sun
, Y.
, and Fu
, J.
, 2011, “A Silicone-Based Stretchable Micropost Array Membrane for Monitoring Live-Cell Subcellular Cytoskeletal Response
,” Lab Chip
, 12
(4
), pp. 731
–740
.28.
Park
, J. S.
, Chu
, J. S.
, Cheng
, C.
, Chen
, F.
, Chen
, D.
, and Li
, S.
, 2004, “Differential Effects of Equiaxial and Uniaxial Strain on Mesenchymal Stem Cells
,” Biotechnol. Bioeng.
, 88
(3
), pp. 359
–368
.29.
Park
, J. S.
, Huang
, N. F.
, Kurpinski
, K. T.
, Patel
, S.
, Hsu
, S.
, Li
, S.
, 2007, “Mechanobiology of Mesenchymal Stem Cells and Their Use in Cardiovascular Repair
,” Front. Biosci.
, 12
, pp. 5098
–5116
.30.
Tzima
, E.
, Irani-Tehrani
, M.
, Kiosses
, W. B.
, Dejana
, E.
, Schultz
, D. A.
, Engelhardt
, B.
, Cao
, G.
, DeLisser
, H.
, and Schwartz
, M. A.
, 2005, “A Mechanosensory Complex That Mediates the Endothelial Cell Response to Fluid Shear Stress
,” Nature
, 437
(7057
), pp. 426
–431
.31.
Zhang
, H.
, Landmann
, F.
, Zahreddine
, H.
, Rodriguez
, D.
, Koch
, M.
, Labouesse
, M. A.
, 2011, “Tension-Induced Mechanotransduction Pathway Promotes Epithelial Morphogenesis
,” Nature
, 471
(7336
), pp. 99
–103
.32.
Lim
, C. T.
, Tan
, E. P. S.
, and Ng
, S. Y.
, 2008, “Effects of Crystalline Morphology on the Tensile Properties of Electrospun Polymer Nanofibers
,” Appl. Phys. Lett.
, 92
(14
), p. 141908
–11
.33.
Creszenzi
, V.
, Mazini
, G.
, Calzolari
, G.
, and Borri
, C.
, 1972, “Thermodynamics of Fusion of Poly-β-Propiolactone and Poly-∈-Caprolactone. Comparative Analysis of the Melting of Aliphatic Polylactone and Polyester Chains
,” Eur. Polym. J.
, 8
(3
), pp. 449
–463
.34.
Thomas
, V.
, Jose
, M.
, Chowdhury
, S.
, Sullivan
, J.
, Dean
, D.
, and Vohra
, Y.
, 2006, “Mechano-Morphological Studies of Aligned Nanofibrous Scaffolds of Polycaprolactone Fabricated by Electrospinning
,” J. Biomater. Sci., Polym. Ed.
, 17
, pp. 969
–984
.35.
Kim
, G. H.
, 2008, “Electrospun PCL Nanofibers With Anisotropic Mechanical Properties as a Biomedical Scaffold
,” Biomed. Mater.
, 3
(2
), p. 025010
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